Nitrocefin is a chromogenic β-lactamase substrate that undergoes colour change from yellow to red as the amide bond in the β-Lactam ring is hydrolyzed by β-lactamase. Nitrocefin undergoes colour changes induced by lactamases produced by Gram-positive and Gram-negative bacteria. Several studies have utilized the colour changing properties of Nitrocefin for the detection of β-lactamase activity from bacterial cell extracts by isoelectric focusing and spectroscopy. Nitrocefin has also been used in studies involving β-lactamase resistant antibiotics.

CAS：41906-86-9
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英文别名：
分子式：C21H16N4O8S2
分子量：516.504
精确质量：516.041
Psa：231.89
Logp：4.1051

UNII：EWP54G0J8F

密度：1.67g/cm3
沸点：872ºC at 760 mmHg
闪点：481.2ºC
折射率：1.749
储存条件：2-8ºC