Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products. It hence has been used as a reagent for the detection of amines and peptides. 1-100µg of protein and down 10pg protein can be detected. This method is found to suffer of high blanks resulting of high rate of hydrolysis due to used excess concentration. Alternative methods are based on ortho-Phthalaldehyde (OPA), Ellman's reagent (DTNB) and epicocconone.

CAS：38183-12-9
中文别名：荧胺;4-苯基螺[呋喃-2(3H),1-二氢异苯并呋喃]-3,3\'-二酮;
英文别名：4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3\'-dione;1\'-phthalan]-3,3\'-dione;4-phenyl-spiro[furan-2,1\'-isobenzofuran]-3,3\'-dione;4-phenylspiro[furan-2(3H),1\'-phthalan]-3,3\'-dione;Fluram(R);4-Phenylspiro[furan-2(3H),1\'-phthalan]-3,3\'-dione;4-phenylspiro[furan-2(3H);Fluorescamine;Spiro[furan-2(3H),1\'(3\'H)-isobenzofuran]-3,3\'-dione,4-phenyl;Fluram;4-phenylspiro(furan-2(3H),1\'(3\'H)-isobenzofuran)-3,3\'-dione;4-Phenylspiro[Furan-2(3H),1-Phthalan]-3,3-Dione;
分子式：C17H10O4
分子量：278.259
精确质量：278.058
Psa：52.6
Logp：2.6502

MDL号：MFCD00005928
EINECS号：253-814-5

外观与性状：白色粉末
密度：1.43g/cm3
熔点：153-157°C(lit.)
闪点：-17°C
折射率：1.686
稳定性：Stable. Incompatible with strong oxidizing agents.
储存条件：2-8ºC

安全说明：S22-S24/25-S36/37-S33-S29-S26-S16-S9-S37/39
危险类别码：R36/37/38
WGK Germany：3
海关编码：2932999099
危险品运输编码：UN 1090 3/PG 2
危险品标志：Xi

用途
氨基酸延生化试剂,荧光检测,在PH=9,室温顺发反应,衍生物具高强度荧光,而试剂本生水解成不带荧光产物。