Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When PI is bound to nucleic acids, the fluorescence excitation maximum is 535nm and the emission maximum is 617nm. Excitation energy can be supplied with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser. Propidium iodide is used as a DNA stain for both flow cytometry, to evaluate cell viability or DNA content in cell cycle analysis, and microscopy to visualise the nucleus and other DNA-containing organelles. It can be used to differentiate necrotic, apoptotic and normal cells.

CAS：25535-16-4
中文别名：3,8-二氨基-5-(二乙氨基丙基)-6;碘化丙锭;
英文别名：EINECS 247-081-0;Propidium diiodide;Propidium iodide solution;3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide;(3,8-diamino-5-(3-diethylmethylamino)propyl)-6-phenylphenanthridinium diiodide;3,8-Diamino-5-[3-(diaethyl-methyl-ammonio)-propyl]-6-phenyl-phenanthridinium,Dijodid;3,8-Diamino-5-(3-Diethylaminopropyl)-6-Phenylphenanthridinium Iodide methIodide;propidium iodine;Propidium iodide;3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide;3,8-diamino-5-[3-(diethyl-methyl-ammonio)-propyl]-6-phenyl-phenanthridinium,diiodide;3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium,diiodide;3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide;
分子式：C27H34I2N4
分子量：668.395
精确质量：668.087
Psa：55.92
Logp：0.1588

BRN号：3843838
PubChem号：24898510
MDL号：MFCD00011921

熔点：220-225°C (dec.)(lit.)
储存条件：2-8ºC

RTECS号：SF7949600
安全说明：S26; S36; S22
WGK Germany：3
危险类别码：R36/37/38
危险品标志：Xi
信号词：Warning
危险性描述：H315; H319; H335; H341
危险标志：GHS07, GHS08
危险性防范说明：P201; P280; P305 + P351 + P338; P308 + P313; P337 + P313