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化合物简介

Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with excitation and emission wavelengths of 495/515nm, respectively, and has the appearance of orange crystals. Calcein self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. It is also used traditionally as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

基本信息

CAS:1461-15-0
中文别名:荧光素配位剂;3,3\'-双(甲胺二乙酸)荧光素;3,3\'-双(甲氨二乙酸)荧光素;3,3\'-双(甲亚胺二乙酸)荧光素;
英文别名:Fluorescein complexon;2-[[7\'-[[bis(carboxymethyl)amino]methyl]-3\',6\'-dihydroxy-3-oxospiro[2-benzofuran-1,9\'-xanthene]-2\'-yl]methyl-(carboxymethyl)amino]acetic acid;Fluorescein-bis(methyliminodiacetic acid);Oftasceinum;Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein;Oftasceina;Fluorexon;Oftasceine;
分子式:C30H26N2O13
分子量:622.533
精确质量:622.143
Psa:231.67
Logp:1.6082

编号系统

MDL号:MFCD00005049
PubChem号:24892279
EINECS号:215-957-1
BRN号:8181068

物化性质

外观与性状:橙色粉末
密度:1.74 g/cm3
沸点:952.7ºC at 760 mmHg
熔点:200 °C
闪点:530ºC
折射率:1.774
水溶解性:insoluble
稳定性:Stable. Incompatible with strong oxidizing agents.
储存条件:Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
蒸汽压:0mmHg at 25°C

安全信息

安全说明:S22-S24/25-S36/37/39-S26
WGK Germany:3
危险类别码:R20/21/22; R36/37/38
海关编码:32041900
危险品标志:Xn

生产方法及用途

用途
络合指示剂用于钙、钢、锶、铜、锰等的测定

合成路线

上游原料

下游产品

图谱

Predict 1H proton NMR


13C NMR : Predict


1H NMR : Predict


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