Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

CAS：1064-48-8
中文别名：萘酚蓝;萘黑10B;酸性黑1;酸性黑1/氨基黑10B;氨基黑10B;
英文别名：Buffalo Black NBR;acidblackh;DermBlueP;aniline blue black;naphtol blue black;KURO401;Black 10B;AMIDO BLACK;Acid Black 1;Aminoschwarz 10B;Naphthol Blue Black;naphthol blue-black;Amido Black Staining;CI NO 0470;ACID BLACK;
分子式：C22H14N6Na2O9S2
分子量：616.491
精确质量：616.006
Psa：272.67
Logp：7.9408

MDL号：MFCD00004017
RTECS号：QJ6196000
EINECS号：213-903-1
BRN号：5374263

外观与性状：暗红色至黑色粉末
密度：1.05g/mLat 20°C
闪点：30°C
水溶解性：10 G/L (25 ºC)
稳定性：Stable. Incompatible with strong oxidizing agents.
储存条件：Store at RT.

RTECS号：QJ6196000
安全说明：S26; S36; S37/39; S45; S36/37/39; S23; S16
危险类别码：R36/37/38
WGK Germany：3
危险品运输编码：UN 2924 3/PG 3
海关编码：3204120000
危险品标志：Xi

生产方法
以 H 酸、对硝基苯胺、苯胺为原料,首先将对硝基苯胺重氮化,于酸性条件下与 H 酸偶合,然后将苯胺重氮化,于碱性条件下与前述偶合产物进行第二次偶合即得产物。经盐析、过滤、干燥、粉碎得成品。。 主要用于羊毛、蚕丝、锦纶及其混纺织物的染色和印花,也可用于拼色
用途
主要用于羊毛、蚕丝、锦纶及其混纺织物的染色和印花,也可用于拼色